Recombinant Polysialic Acid antibody

Catalog No. ABIN5668052

Cited in 1 publication. The Mouse Monoclonal anti-Polysialic Acid antibody (Clone 735) (ABIN5668052) specifically detects Polysialic Acid in FACS, IHC, IP and WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Recombinant Polysialic Acid antibody (ABIN5668052)

Target: Polysialic Acid

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Clone: 735

Product Details

Purpose: Anti-Polysialic acid [735], Mouse IgG2a, kappa

Specificity: Recognises Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), which shares molecular epitopes with homopolymers of α2,8-linked sialic acid of Escherichia coli K1.

Cross-Reactivity: Mouse, Rat

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: Homopolymers of alpha2,8-linked sialic acid of Escherichia coli K1.

Isotype: IgG2a kappa

Application Details

Application Notes: Clone 735 was used for immunostaining and immunoprecipitation in an experimental setup involving antigen preparation from PBMC (Peripheral Blood Mononuclear Cells) (Husmann et al., 1989, PMID: 2529126). This antibody recognises an unusual postranslational modification on neural cell adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and development of organs such as the kidney (studied in rats, Lackie et al., 1990). 735 was used for Western blot analysis of PSA-NCAM (Polysialylated Neural Cell Adhesion Molecule) (Charter et al., 2000, PMID: 11030751). In addition to various studies on human cells, the antibody, has also been used the study the fine-composition of poly-sialic acid composition in mice (Galuska et al., 2006).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

References

Tytgat, Lin, Levasseur, Tomek, Rutschmann, Mock, Liebscher, Terasaka, Azuma, Wetter, Bachmann, Hilvert, Aebi, Keys: "Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies." in: Nature communications, Vol. 10, Issue 1, pp. 5403, (2020) (PubMed).

Target Details

Target: Polysialic Acid

Background: PSA, N-CAM, neural cell adhesion molecule, polySA