Recombinant p53 antibody (AA 371-380)

Catalog No. ABIN5668086

This Rabbit Chimeric antibody specifically detects p53 in WB, ELISA, IF, IHC (p), IP, IHC (fro) and in vivo. It exhibits reactivity toward Human, Mouse, Rat, Monkey and Rabbit.

Quick Overview for Recombinant p53 antibody (AA 371-380) (ABIN5668086)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Monkey, Rabbit

Host: Rabbit

Clonality: Chimeric

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), In vivo Studies (in vivo)

Clone: PAb421

Product Details anti-p53 Antibody

Binding Specificity: AA 371-380

Purpose: Anti-p53 [PAb421], Rabbit IgG, kappa

Specificity: Recognises the mammalian mutant and wild-type p53 protein and reacts with an epitope between amino acid residues 370 and 378 (near the C-terminus of the protein), corresponding to the sequence "KKGQSTSRHK".

Cross-Reactivity: Monkey, Mouse, Rabbit, Rat

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: Synthetic peptide corresponding to aa 371-380 of human p53.

Isotype: IgG kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Background: Tumor suppressor p53, Cellular tumor antigen p53

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect