Recombinant TUBB antibody

Catalog No. ABIN5668108

The Mouse Chimeric anti-TUBB antibody is suitable to detect TUBB in samples from Human. It has been validated for WB, IF and ELISA.

Quick Overview for Recombinant TUBB antibody (ABIN5668108)

Target: TUBB (Tubulin, beta (TUBB))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Chimeric

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Clone: S11B

Product Details anti-TUBB Antibody

Purpose: Anti-Beta-Tubulin [S11B], Mouse IgG1, lambda

Specificity:

This antibody is specific for human beta-tubulin (no cross-reactivity with alpha-tubulin). Tubulin is the major constituent of microtubules. The alpha chain has a non-exchangeable GTP-binding site. Tubulin is the major constituent of microtubules. The beta-chain has an exchangeable GTP-binding site.

Application Notes original Clone: This antibody's original format (human scFv) was successfully used in a Western blot, and its anti-myc (9E10) complexed version was successfully used for in vitro immunofluorescence staining of β-tubulin in HeLa cells (Nizak et al., 2003, PMID: 14617357).

Characteristics: This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: The original antibody was generated via phage display from the Griffin.1 library by screening against Microtubule-Binding Proteins (MBPs) derived from HeLa S3 cells.

Isotype: IgG1, lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Human

OriginalFormat: scFv

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: Beta-Tubulin

Background: TUBB, β-tubulin

UniProt: Q13509

Pathways: Microtubule Dynamics, M Phase