Recombinant EPH Receptor A2 antibody (AA 183-510)
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- Target See all EPH Receptor A2 (EPHA2) Antibodies
- EPH Receptor A2 (EPHA2)
- Antibody Type
- Recombinant Antibody
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Binding Specificity
- AA 183-510
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Chimeric
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Conjugate
- This EPH Receptor A2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Agonist (Agon), Internalization Assay (INA)
- Specificity
- 1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM, Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, EphA2, and EphA4, or any EphB receptor family members.
- Characteristics
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OriginalSpeciesName: Human
OriginalFormat: IgG1
- Purification
- Purified antibody.
- Immunogen
- 1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.
- Clone
- 1C1
- Isotype
- IgG kappa
- Top Product
- Discover our top product EPHA2 Primary Antibody
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- Application Notes
- This antibody binds to human Ephrin type-A receptor 2, a receptor tyrosine kinase that binds to membrane-bound ephrin-A ligands residing on adjacent cells, triggering contact-dependent signaling into neighboring cells. Eph2A is involved in pattern formation during development, angiogenesis and osteoblastogenesis. Eph2A has also been found to be expressed in a variety of cancers. This antibody acts as an agonist of Eph2A signaling. The agonist properties of 1C1 is due to ligand mimicry by VDR3. After cell binding, the antibody is able to induce rapid tyrosine phosphorylation, internalization, and degradation of the EphA2 receptor.
- Comment
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This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
- Restrictions
- For Research Use only
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- Buffer
- PBS with 0.02 % Proclin 300.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Target
- EPH Receptor A2 (EPHA2)
- Alternative Name
- Ephrin Type A receptor 2 (EPHA2 Products)
- Synonyms
- ARCC2 antibody, CTPA antibody, CTPP1 antibody, CTRCT6 antibody, ECK antibody, epha2 antibody, MGC89505 antibody, AW545284 antibody, Eck antibody, Myk2 antibody, Sek-2 antibody, Sek2 antibody, id:ibd5152 antibody, rtk6 antibody, G42 antibody, XE10 antibody, epha2-A antibody, EPH receptor A2 antibody, Eph receptor A2 antibody, epoxide hydrolase antibody, eph receptor A2 a antibody, EPH receptor A2 L homeolog antibody, EPHA2 antibody, Epha2 antibody, epha2 antibody, ephA2 antibody, epha2a antibody, epha2.L antibody
- Background
- EphA2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK
- UniProt
- P29317
- Pathways
- RTK Signaling
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