Recombinant EPH Receptor A2 antibody (AA 183-510)

Catalog No. ABIN5668240

This anti-EPH Receptor A2 antibody is a Rabbit Chimeric antibody detecting EPH Receptor A2 in WB, ELISA, FACS, IF, Agon and INA. Suitable for Human.

Quick Overview for Recombinant EPH Receptor A2 antibody (AA 183-510) (ABIN5668240)

Target: EPH Receptor A2 (EPHA2)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This EPH Receptor A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Agonist (Agon), Internalization Assay (INA)

Clone: 1C1

Product Details anti-EPH Receptor A2 Antibody

Binding Specificity: AA 183-510

Purpose: Anti-Human Ephrin Type A receptor 2 [1C1], Rabbit IgG, kappa

Specificity: 1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM, Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, and EphA4, or any EphB receptor family members. It is reported to cross react with mouse EphA2.

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: 1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.

Isotype: IgG kappa

Application Details

Application Notes: This antibody binds to human Ephrin type-A receptor 2, a receptor tyrosine kinase that binds to membrane-bound ephrin-A ligands residing on adjacent cells, triggering contact-dependent signaling into neighboring cells. Eph2A is involved in pattern formation during development, angiogenesis and osteoblastogenesis. Eph2A has also been found to be expressed in a variety of cancers. This antibody acts as an agonist of Eph2A signaling. The agonist properties of 1C1 is due to ligand mimicry by VDR3. After cell binding, the antibody is able to induce rapid tyrosine phosphorylation, internalization, and degradation of the EphA2 receptor.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Alternative Name: Ephrin Type A receptor 2

Background: EphA2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK

UniProt: P29317

Pathways: RTK Signaling