Recombinant CD4 antibody

Catalog No. ABIN5668250

This Rabbit Chimeric antibody specifically detects CD4 in FACS, IP, IF, IHC (p), BP and Inh. It exhibits reactivity toward Human, Rhesus Monkey and Cynomolgus.

Quick Overview for Recombinant CD4 antibody (ABIN5668250)

Target: CD4

Antibody Type: Recombinant Antibody

Reactivity: Human, Rhesus Monkey, Cynomolgus

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Peptide (BP), Inhibition (Inh)

Clone: MT310

Product Details anti-CD4 Antibody

Purpose: Anti-CD4 [MT310], Rabbit IgG, kappa

Specificity: This antibody is specific for the CD4 extracellular domain D1 (binding the HIV-1 gp120-binding site).

Cross-Reactivity: Cynomolgus, Rhesus Monkey

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Isotype: IgG kappa

Application Details

Application Notes: This antibody strongly inhibits HIV-1 gp120-binding to CD4, which blocks HIV infectivity of CD4⁺ T lymphocytes, as well as the binding of MHCII, which results in the block of T-cell activation (possibly leading to anergy induction) (Qin et al., 2001, PMID: n/a). Additionally, antibody binding to CD4 leads to GM-CSF, TNF-α and IL-10 induction. (TNF-α induction only occurs after 20 hours.)

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: Leu3/T3, T-cell differentiation antigen, Ly-4, L3T4, T-cell surface antigen T4/Leu-3

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events