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Recombinant His Tag antibody

This anti-His Tag antibody is a Mouse Monoclonal antibody detecting His Tag in WB, ELISA and FACS. Suitable for .

(1 µg/ml, right panel) staining of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts. (Recombinant His Tag antibody)

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Catalog No. ABIN5668255

Quick Overview for Recombinant His Tag antibody (ABIN5668255)

Target

See all His Tag Antibodies

His Tag

Antibody Type

Recombinant Antibody

Reactivity

Please inquire

Host

106
65
11
10
9
8
2
1

Mouse

Clonality

Monoclonal

Conjugate

This His Tag antibody is un-conjugated

Application

167
115
40
32
31
30
28
16
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13
13
13
7
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5
4
4
4
3
3
2
2
1
1
1
1
1

Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone

3D5

Discover our top product His Tag Primary Antibody

Purpose

Anti-His tag (C-term) [3D5], Mouse IgG1, kappa

Specificity

This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.

Characteristics

Original Species of Ab: Mouse
Original Format of Ab: IgG2b

Purification

Protein A affinity purified

Immunogen

Purified his tagged Fab fragment.

Isotype

IgG1 kappa
anti-His Tag antibody
Reactivity: Human WB Host: Mouse Monoclonal 6AT18 unconjugated

anti-His Tag antibody
WB, ELISA, IHC, DB Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-His Tag antibody
WB, ELISA, IP, IF, FACS Host: Mouse Monoclonal 6G2A9 unconjugated

anti-His Tag antibody
WB, ELISA, IP Host: Mouse Monoclonal 33D10-D2-G8 unconjugated

anti-His Tag antibody
Reactivity: Human WB Host: Rabbit Polyclonal RB19960 unconjugated

anti-His Tag antibody
WB, ELISA, IP, DB, ISt Host: Mouse Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (Cy3)
WB, ELISA, FACS, FM, DB, FLISA Host: Mouse Monoclonal 33D10-D2-G8 Cy3

anti-His Tag antibody (DyLight 800)
WB, ELISA, FM, DB, FLISA Host: Mouse Monoclonal 33D10-D2-G8 DyLight 800

anti-His Tag antibody (DyLight 649)
WB, ELISA, FM, DB, FLISA Host: Mouse Monoclonal 33D10-D2-G8 DyLight 649

Application Notes

This antibody was successfully used for ELISA, WB, and FACS. Monomeric and dimeric scFv versions of the antibody were generated and described (Lindner et al., 1997, PMID: 8994661). The scFv version of this antibody was engineered into a lentiviral vector system to confer specificity for cells displaying the His tag on their surface, enabling targeted gene transfer into HT1080-anti-His cells (Friedel et al., 2015, PMID: 25715658). This antibody was used in FC to detect Canine IL-17A in Canine IL-17A-transfected HEK293 A cells (Akiyama et al., 2019, PMID: 30885301).

Comment

This is a reformatted mouse IgG1 antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

PBS with 0.02 % Proclin 300.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Target

His Tag

Target Type

Tag

Background

Polyhistidine tag

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