NGEF antibody (C-Term)

Catalog No. ABIN5673216

This Rabbit Polyclonal antibody specifically detects NGEF in WB. It exhibits reactivity toward Mouse.

Quick Overview for NGEF antibody (C-Term) (ABIN5673216)

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NGEF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NGEF Antibody

Binding Specificity: C-Term

Sequence: GWFPSSMTEE ILNPKIRSQN LKECFRVHKM EDPQRSQNKD RRKLGSRNRQ

Purification: Affinity purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of mouse NGEF

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NGEF

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Alternative Name: NGEF

Background: Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.

Alias Symbols: Tims2, ephexin

Protein Size: 620

Gene ID: 53972

NCBI Accession: NM_001111314, NP_001104784

Pathways: Neurotrophin Signaling Pathway