SMAD5 antibody (pSer463, pSer465)

Catalog No. ABIN5675103

This Rabbit Polyclonal antibody specifically detects SMAD5 in WB, ELISA, IF (cc), IF (p), ICC, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SMAD5 antibody (pSer463, pSer465) (ABIN5675103)

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SMAD5 Antibody

Binding Specificity: pSer463, pSer465

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human SMAD5 around the phosphorylation site of Ser463 + Ser465

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: SMAD5 (

Background:

Synonyms: Mothers against decapentaplegic homolog 5, SMAD5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5, SMAD 5, Smad5, hSmad5

Background: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Gene ID: 4090

UniProt: Q99717