Collagen IV antibody

Catalog No. ABIN5680242

This Mouse Monoclonal antibody specifically detects Collagen IV in WB, IHC (fro) and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Collagen IV antibody (ABIN5680242)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: COL 94

Product Details anti-Collagen IV Antibody

Specificity: Human Collagen IV, no cross-reactivity with other Collagen types, Vitronectin, Fibronectin or Chondroitinsulfate Human and other primates, no reactivity in chicken

No Cross-Reactivity: Cat, Chicken, Goat, Rabbit, Rat, Sheep

Cross-Reactivity (Details): Human and other primates (no reactivity in chicken, cat, rabbit, rat, sheep and goat)

Characteristics: Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human Collagen IV, no cross-reactivity with other Collagen types, Vitronectin, Fibronectin or Chondroitinsulfate.

Purification: Antibody solution

Immunogen: Purified human Collagen IV

Isotype: IgG1

Application Details

Application Notes: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human skin, placenta or lens capsule,IHC(C,P), WB,60 min at RT,(liquid conc.) 1:10 - 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: stabilizing phosphate buffer  pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 μL working solution / test)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV