CPY antibody (Biotin)

Catalog No. ABIN568127

The Rabbit Polyclonal anti-CPY antibody has been validated for WB, IF, IP, EIA, DB, ID and RIA. It is suitable to detect CPY in samples from Saccharomyces cerevisiae.

Quick Overview for CPY antibody (Biotin) (ABIN568127)

Target: CPY (Carboxypeptidase Y (CPY))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPY antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)

Product Details anti-CPY Antibody (Biotin)

Specificity: The antibody recognizes Carboxypeptidase Y from Baker's Yeast. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Molar Ratio: Biotin/IgG ~1.8

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Carboxypeptidase Y isolated and purified from Baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for CPY

Target: CPY (Carboxypeptidase Y (CPY))

Alternative Name: Carboxypeptidase Y

Background: Carboxypeptidase Y is involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.Synonyms: Carboxypeptidase YSCY, PRC1

Gene ID: 855343

NCBI Accession: NP_014026

UniProt: P00729