Isocitrate Dehydrogenase antibody
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- Target See all Isocitrate Dehydrogenase (IDH) Antibodies
- Isocitrate Dehydrogenase (IDH)
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Reactivity
- Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Isocitrate Dehydrogenase antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)
- Purification
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
- Immunogen
- Isocitrate Dehydrogenase isolated and purified from Porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
- Top Product
- Discover our top product IDH Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Reconstitution
- Restore by adding 1.0 mL sterile distilled water
- Concentration
- 10 mg/mL
- Buffer
- PBS, pH 7.2 without preservatives and foreign proteins
- Preservative
- Without preservative
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.
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- Target
- Isocitrate Dehydrogenase (IDH)
- Alternative Name
- Isocitrate Dehydrogenase / IDH (IDH Products)
- Background
- Isocitrate dehydrogenase is an enzyme involved in the citric acid cycle. It is 416 amino acids long with a molecular weight of approximately 45 kDa. Isocitrate dehydrogenase enzymes catalyze the oxidative decarboxylation of isocitrate to produce alfa ketoglutarate. The human genome has 5 IDH genes coding for 3 IDH enzymes. The IDH1 and IDH2 require nicotinamide adeninedinucleotide phosphate (NADP) as co-substrate, whereas IDH3 require nicotinamide adenine dinucleotide (NAD). The IDH2 and 3 are localized in mitochondria and are actively involved in the citric acid cycle (TCA) for energy production in contrast, IDH1 is localized in cytoplasm and peroxisomes where it generates NADPH, reduced form of NADP for biosynthetic and other types of reaction. Since alfa KG and NADPH both are intermediately substrate for a number of cellular process, which allows the possibility of oncogenic or tumor suppressive activities of IDH1.Synonyms: Cytosolic NADP-isocitrate dehydrogenase, ICDH, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD, cytoplasmic Isocitrate dehydrogenase [NADP]
- Gene ID
- 9823
- UniProt
- P20304
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