PKM2 antibody (Biotin)
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- Target See all PKM2 Antibodies
- PKM2 (Pyruvate Kinase M2 (PKM2))
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Reactivity
- Rabbit
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This PKM2 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Radioimmunoassay (RIA), Immunodiffusion (ID)
- Specificity
- Biotin-conjugated IgG fraction of polyclonal sheep antiserum to pyruvate kinase from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
- Characteristics
- Molar Ratio: Biotin/IgG ~9.1
- Purification
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
- Immunogen
- Pyruvate kinase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedur
- Isotype
- IgG
- Top Product
- Discover our top product PKM2 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Restore by adding 1.0 mL of sterile distilled water
- Concentration
- 10,0 mg/mL
- Buffer
- PBS, pH 7.2 without preservatives and foreign proteins
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.
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- Target
- PKM2 (Pyruvate Kinase M2 (PKM2))
- Alternative Name
- PKM2 (PKM2 Products)
- Background
- Synonyms: CTHBP, Cytosolic thyroid hormone-binding protein, M2-PK, OIP-3, OIP3, Opa-interacting protein 3, PK2, PK3, PKM, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle, Pyruvate kinase muscle isozyme, THBP1
- Gene ID
- 100008676
- NCBI Accession
- NP_001182573
- UniProt
- P11974
- Pathways
- Warburg Effect
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