PKM2 antibody (Biotin)

Catalog No. ABIN568419

This anti-PKM2 antibody is a Sheep Polyclonal antibody detecting PKM2 in WB, IF, IP, EIA, DB, RIA and ID. Suitable for Rabbit.

Quick Overview for PKM2 antibody (Biotin) (ABIN568419)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: This PKM2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Radioimmunoassay (RIA), Immunodiffusion (ID)

Product Details anti-PKM2 Antibody (Biotin)

Specificity: Biotin-conjugated IgG fraction of polyclonal sheep antiserum to pyruvate kinase from rabbit muscle. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Characteristics: Molar Ratio: Biotin/IgG ~9.1

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Pyruvate kinase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedur

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10,0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2

Background: Synonyms: CTHBP, Cytosolic thyroid hormone-binding protein, M2-PK, OIP-3, OIP3, Opa-interacting protein 3, PK2, PK3, PKM, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle, Pyruvate kinase muscle isozyme, THBP1

Gene ID: 100008676

NCBI Accession: NP_001182573

UniProt: P11974

Pathways: Warburg Effect