Urate Oxidase antibody
The Rabbit Polyclonal anti-Urate Oxidase antibody has been validated for WB, IP, IF, EIA, DB, ID and RIA. It is suitable to detect Urate Oxidase in samples from Pig.
Quick Overview for Urate Oxidase antibody (ABIN568442)
Target
See all Urate Oxidase (UOX) Antibodies
Urate Oxidase (UOX)
Reactivity
Pig
Host
Rabbit
Clonality
Polyclonal
Conjugate
This Urate Oxidase antibody is un-conjugated
Application
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)
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Specificity
- IgG fraction of polyclonal rabbit antiserum to uricase from porcine liver. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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Purification
- Ammonium Sulphate Precipitation and Ion Exchange Chromatography
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Immunogen
- Uricase is isolated and purified from porcine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Isotype
- IgG
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anti-Urate Oxidase (UOX) (AA 21-120) antibody
UOX Reactivity: Mouse WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Reconstitution
- Restore by adding 1.0 mL of sterile distilled water
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Concentration
- 10,0 mg/mL
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Buffer
- PBS, pH 7.2 without preservatives and foreign proteins
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Preservative
- Without preservative
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -20 °C for longer.
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