GOT1 antibody

Catalog No. ABIN568461

This anti-GOT1 antibody is a Rabbit Polyclonal antibody detecting GOT1 in WB, IP, IF, EIA, DB, ID and RIA. Suitable for Pig.

Quick Overview for GOT1 antibody (ABIN568461)

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)

Product Details anti-GOT1 Antibody

Specificity: Glutamate-Oxaloacetate Transaminase from Porcine heart. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Glutamate-Oxaloacetate Transaminase isolated and purified from Porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: GOT1

Background: Glutamate oxaloacetate transaminase is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80 % of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.Synonyms: Aspartate aminotransferase, Glutamate oxaloacetate transaminase 1, Transaminase A

Gene ID: 396967

NCBI Accession: NP_999092

UniProt: P00503

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process