UGDH antibody

Catalog No. ABIN568499

The Rabbit Polyclonal anti-UGDH antibody is suitable to detect UGDH in samples from Cow. It has been validated for WB, IF, IP, EIA, DB, ID and RIA.

Quick Overview for UGDH antibody (ABIN568499)

Target: UGDH (UDP-Glucose 6-Dehydrogenase (UGDH))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UGDH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Radioimmunoassay (RIA)

Product Details anti-UGDH Antibody

Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: Uridine-5’-Diphosphoglucose Dehydrogenase isolated and purified from Bovine liver. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 1.0 mL of sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Target Details for UGDH

Target: UGDH (UDP-Glucose 6-Dehydrogenase (UGDH))

Alternative Name: UGDH

Background: UGDH is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis.Synonyms: UDP-Glc dehydrogenase, UDP-GlcDH, UDP-glucose 6-dehydrogenase, UDPGDH

Gene ID: 281564

NCBI Accession: NP_776636

UniProt: P12378

Pathways: Glycosaminoglycan Metabolic Process