C3 antibody (FITC)

Catalog No. ABIN568516

This Goat Polyclonal antibody specifically detects C3 in IP and ID. It exhibits reactivity toward Dog.

Quick Overview for C3 antibody (FITC) (ABIN568516)

Target: C3 (Complement Component 3 (C3))

Reactivity: Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This C3 antibody is conjugated to FITC

Application: Immunoprecipitation (IP), Immunodiffusion (ID)

Product Details anti-C3 Antibody (FITC)

Characteristics: Absorption / Emission: 492 nm / 515 nm
Molar Ratio: Fluorescein/IgG ~1.7

Purification: The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and Double Radial Immunodiffusion. Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: C3c is isolated and purified from pooled normal Dog serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1 mL of sterile distilled water.

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid Repeated thawing and freezing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement C3

Background: C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain.Synonyms: CPAMD1, Complement component 3

Gene ID: 9606

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling