C5 antibody (FITC)

Catalog No. ABIN568577

The Goat Polyclonal anti-C5 antibody is suitable to detect C5 in samples from Human. It has been validated for IP, IHC (fro), EIA, ID and IF.

Quick Overview for C5 antibody (FITC) (ABIN568577)

Target: C5 (Complement Component 5 (C5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This C5 antibody is conjugated to FITC

Application: Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunofluorescence (IF)

Product Details anti-C5 Antibody (FITC)

Specificity: The reactivity of the antiserum is restricted to C5. In immunoelectrophoresis and Radial Immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C5 protein. No reaction is obtained with any other plasma protein component or serum. The antiserum does not cross react with any other component of human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Characteristics: Absorption / Emission: 492 nm / 515 nm
Molar Ratio: Fluorescein/IgG ~1.4

Purification: The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by Immunoelectrophoresis and Double Radial Immunodiffusion.

Immunogen: C5 protein is isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1 mL of sterile distilled water.

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid Repeated thawing and freezing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for C5

Target: C5 (Complement Component 5 (C5))

Alternative Name: Complement C5

Background: C5 component of human complement is a plasma protein consisting of two polypeptide chains, alpha, beta, bound by disulphide bonds. It has a molecular weight of 190,000. The activated protein forms with the complement factors C6-C9 the membrane attack complex.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, CPAMD4, Complement component 5

Gene ID: 727

NCBI Accession: NP_001726

UniProt: P01031

Pathways: Complement System, Carbohydrate Homeostasis