CCL26 antibody
Quick Overview for CCL26 antibody (ABIN5687579)
Target
See all CCL26 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Purification
- Anti-hEotaxin-3 specific antibody was purified by affinity chromatography employing immobilized hEotaxin-3 matrix.
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Immunogen
- Produced from sera of goats pre-immunized with highly pure (>98%) recombinant human Eotaxin-3.
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Application Notes
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Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of human eotaxin-3 (100.00 ng/mL), a concentration of 5.0 - 7.0 μ,g/mL of this antibody is required.
ELISA:
To detect human eotaxin-3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant human eotaxin-3.
Sandwich: To detect hEotaxin-3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Eotaxin-3 (XP-5127Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin-3.
Western Blot:
To detect human eotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant human eotaxin-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Storage
- -20 °C
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Storage Comment
- Eotaxin-3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- CCL26 (Chemokine (C-C Motif) Ligand 26 (CCL26))
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Alternative Name
- Eotaxin-3
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Gene ID
- 10344
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UniProt
- Q9Y258
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Pathways
- Regulation of Actin Filament Polymerization
Target
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