Thrombopoietin antibody

Catalog No. ABIN5687619

This Goat Polyclonal antibody specifically detects Thrombopoietin in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for Thrombopoietin antibody (ABIN5687619)

Target: Thrombopoietin (THPO)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Thrombopoietin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-Thrombopoietin Antibody

Purification: Anti-hTPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTPO.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTPO (5 ng/mL), a concentration of 4.2 - 6.3 μ,g/mL of this antibody is required.

ELISA:

To detect hTPO by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTPO.

Sandwich:

To detect hTPO by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TPO (XP-5288Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.

Western Blot:
To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: TPO antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Thrombopoietin

Target: Thrombopoietin (THPO)

Alternative Name: Thrombopoietin (TPO)

Background: Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Gene ID: 7066

UniProt: P40225

Pathways: JAK-STAT Signaling, Hormone Activity