IL1A antibody

Catalog No. ABIN5687646

This Goat Polyclonal antibody specifically detects IL1A in WB, ELISA and Neut. It exhibits reactivity toward Rat.

Quick Overview for IL1A antibody (ABIN5687646)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IL1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL1A Antibody

Purification: Anti-Rat IL-1alpha specific antibody was purified by affinity chromatography employing immobilized Rat IL-1alpha matrix.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-1alpha (Rat IL-1alpha).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-1α, (50pg/mL), a concentration of 0.011-0.017 μ,g/mL of this antibody is required.

ELISA:

To detect Rat IL-1α, by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat IL-1α,.

Sandwich:

To detect Rat IL-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-1α, (XP-5176Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1α,.

Western Blot:
To detect Rat IL-1α, by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1α, is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1a

Gene ID: 24493

UniProt: P16598

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints