IL-3 beta antibody

Catalog No. ABIN5687647

The Goat Polyclonal anti-IL-3 beta antibody has been validated for WB, ELISA and Neut. It is suitable to detect IL-3 beta in samples from Rat.

Quick Overview for IL-3 beta antibody (ABIN5687647)

Target: IL-3 beta (Interleukin 3 beta (IL-3 beta))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-3 beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-3 beta Antibody

Purification: Anti-rat IL-3β, specific antibody was purified by affinity chromatography employing immobilized hIL-3β, matrix.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-3β, (rat IL-3 beta).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-3β, (150 ng/mL), a concentration of 0.68 - 1.03 μ,g/mL of this antibody is required.

ELISA:

To detect Rat IL-3β, by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3β,..

Sandwich:

To detect Rat IL-3β, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IL-3β, (XP-5187Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-3β,.

Western Blot:

To detect Rat IL-3β, by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3β, is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-3B antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-3 beta

Target: IL-3 beta (Interleukin 3 beta (IL-3 beta))

Alternative Name: IL-3B

Background: IL-3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells

Gene ID: 24495

UniProt: P97688