The immunizing peptide represents part of the extracellular domain.
Purification
OSMR Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
OSMR Antibody was raised against synthetic peptide QIHGEQLDPHVT found at an internal region of OSMR.
OSM
Reactivity: Rat
ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 125 kDa band observed in lysates of cell lines HeLa, A549 and U937 (calculated MW of 111 kDa according to NP_003990.1). Recommended concentration: 0.1-0.3μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
OSMR is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and store at -20°C. Minimize freezing and thawing.