Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10.0 ng/mL), a concentration of <0.2 μ,g/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100uL/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with our biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 μ,g/mL.
Western Blot:
To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.
Restrictions
For Research Use only
Format
Lyophilized
Storage
4 °C,-20 °C
Storage Comment
VEGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.