NGFB antibody

Catalog No. ABIN5688734

The Mouse Monoclonal anti-NGFB antibody (Clone M85-2B) (ABIN5688734) specifically detects NGFB in WB, ELISA and Neut. The antibody is reactive with Human samples.

Quick Overview for NGFB antibody (ABIN5688734)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: M85-2B

Product Details anti-NGFB Antibody

Purification: This IgG2bK antibody was purified from cell culture by Protein G affinity chromatography.

Immunogen: beta-NGF antibody is produced in mice using highly pure (>98%) recombinant human beta-NGF as the immunizing antigen.

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hB-NGF (10.0 ng/mL), a concentration of 2.7 - 4.0 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 200 pg/mL of recombinant human β,-NGF when used with our biotinylated antigen affinity purified anti-human b-NGF as the detection antibody at a concentration of approximately 0.5-1.0 μ,g/mL.

Western Blot:

To detect hβ,-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ,-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: 4 °C,-20 °C

Storage Comment: beta-NGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Target Details for NGFB

Target: NGFB (Nerve Growth Factor beta (NGFB))

Alternative Name: bNGF

Gene ID: 4803

UniProt: P01138

Pathways: NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size