SYN1 antibody (pSer9)

Catalog No. ABIN5688911

This anti-SYN1 antibody is a Rabbit Polyclonal antibody detecting SYN1 in WB and IHC. Suitable for Human, Rat, Mouse and Xenopus laevis.

Quick Overview for SYN1 antibody (pSer9) (ABIN5688911)

Target: SYN1 (Synapsin I (SYN1))

Reactivity: Human, Rat, Mouse, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SYN1 Antibody

Binding Specificity: pSer9

Specificity: Synapsin antibody is specific for Synapsin I phosphorylated at Ser9.

Purification: Affinity Purified

Immunogen: Synapsin (Ser9) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Ser9 of Rat Synapsin I.

Application Details

Application Notes: Immunolabeling of the 78k Synapsin I doublet in a Western blot of rat brain homogenate is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. The antibody also weakly labels Synapsin II (55k) which has a similar phosphorylation site to that of Ser9 on Synapsin I. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse, rat and Xenopus have 100 % amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: Synapsin

Background: Synapsin I plays a key role in synaptic plasticity in brain. This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation. Thus synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation. Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I. Phosphorylation of this site is thought to regulate synaptic vesicle function.

Molecular Weight: 78 kDa

Gene ID: 281510

UniProt: P17599