IL-13 antibody

Catalog No. ABIN5689030

The Rabbit Polyclonal anti-IL-13 antibody has been validated for WB, ELISA and Neut. It is suitable to detect IL-13 in samples from Human.

Quick Overview for IL-13 antibody (ABIN5689030)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-13 Antibody

Purification: Anti-hIL-13 specific antibody was purified by affinity chromatography employing immobilized hIL-13 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-13 (human Interleukin-13).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-13 (2.0 ng/mL), a concentration of 0.12 - 0.15 μ,g/mL of this antibody is required.

ELISA:

To detect hIL-13 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13.

Sandwich

To detect hIL-13 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-13 (XP-5167Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-13.

Western Blot:

To detect hIL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-13 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: IL-13

Background: Human IL13 was originally identified by differential screening of an anti CD28 activated human peripheral blood mononuclear cell cDNA library as an induction specific novel cytokine. It was also isolated from cDNA libraries of human T cell clones using the murine IL13 (P600) cDNA as a probe. Human IL13, a pleiotropic cytokine, is produced by activated Th0, Th1 like, Th2 like, and CD8 T cells. The gene for human IL13 maps to chromosome 5 and is closely linked to the genes for IL3, IL4, IL5, and GMCSF.

Gene ID: 3596

UniProt: P35225

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport