M-CSF/CSF1 antibody

Catalog No. ABIN5689065

This Rabbit Polyclonal antibody specifically detects M-CSF/CSF1 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for M-CSF/CSF1 antibody (ABIN5689065)

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This M-CSF/CSF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-M-CSF/CSF1 Antibody

Purification: Anti-hM-CSF specific antibody was purified by affinity chromatography employing immobilized hM-CSF matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hM-CSF (human M-CSF).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/mL), a concentration of 0.02 0.03 μ,g/mL of this antibody is required.

ELISA:

To detect hM-CSF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF.

Sandwich:

To detect hM-CSF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human M-CSF (XP-5227Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hM-CSF.

Western Blot:
To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: M-CSF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for M-CSF/CSF1

Target: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Alternative Name: M-CSF

Background: Macrophage CSF, also known as CSF1, is produced by monocytes, fibroblasts and endothelial cells. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osetoclasts. Natural human MCSF is a dimeric glycoprotein of 70-90 kD molecular weight, existing in multiple glycosylation forms. It binds to a 165 kD glycoprotein of the receptor tyrosine kinase subclass III, a family that includes the receptors for platelet derived growth factor (PDGF) and stem cell factor (SCF).

Gene ID: 1435

UniProt: P09603

Pathways: RTK Signaling