Oncostatin M antibody

Catalog No. ABIN5689094

Product Details anti-Oncostatin M Antibody

Target: Oncostatin M (OSM)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Oncostatin M antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Purification: Anti-hOncostatin-M specific antibody was purified by affinity chromatography employing immobilized hOncostatin-M matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hOncostatin-M (human Oncostatin-M).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hOncostatin-M (10 ng/mL), a concentration of 0.100 - 0.175 μ,g/mL of this antibody is required.

ELISA:

To detect hOncostatin-M by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hOncostatin-M.

Sandwich:

To detect hOncostatin M by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Oncostatin M (XP-5252Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOncostatin M.

Western Blot:
To detect hOncostatin-M by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOncostatin-M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: OncostatinM antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for Oncostatin M

Target: Oncostatin M (OSM)

Alternative Name: OncostatinM (OSM Products)

Synonyms: OSM antibody, OncoM antibody, oncostatin M antibody, OSM antibody, Osm antibody

Gene ID: 5008

UniProt: P13725

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion