IL1A antibody

Catalog No. ABIN5689137

This Rabbit Polyclonal antibody specifically detects IL1A in WB, ELISA and Neut. It exhibits reactivity toward Mouse.

Quick Overview for IL1A antibody (ABIN5689137)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL1A Antibody

Purification: Anti-mIL-1 alpha specific antibody was purified by affinity chromatography employing immobilized mIL-1 alpha matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1-alpha (murine IL-1 alpha).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1 alpha (0.05 ng/mL), a concentration of 0.03 - 0.05 μ,g/mL of this antibody is required.

ELISA:

To detect mIL-1 alpha by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1 alpha.

Sandwich:

To detect mIL-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-1α, (XP-5175Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1α,.

Western Blot:

To detect mIL-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: IL-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1a

Gene ID: 16175

UniProt: P01582

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints