CCL3 antibody

Catalog No. ABIN5689165

The Rabbit Polyclonal anti-CCL3 antibody is suitable to detect CCL3 in samples from Mouse. It has been validated for WB, ELISA and Neut.

Quick Overview for CCL3 antibody (ABIN5689165)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL3 Antibody

Purification: Anti-mMIP-1-alpha specific antibody was purified by affinity chromatography employing immobilized mMIP-1-alpha matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1-alpha (murine Macrophage Inflammatory Protein-1-alpha).

Application Details

Application Notes: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mMIP-1-alpha (10 ng/mL), a concentration of 2.0 - 5.0 μ,g/mL of this antibody is required.

ELISA:

To detect mMIP-1-alpha by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mMIP-1-alpha.

Sandwich:

To detect hMIP-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine MIP-1α, (XP-5234Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-1α,.

Western Blot:

To detect mMIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: MIP-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Alternative Name: MIP-1a

Background: MIP1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.

Gene ID: 20302

UniProt: P10855

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy