Paxillin antibody (AA 29-33)

Catalog No. ABIN5689331

This anti-Paxillin antibody is a Rabbit Polyclonal antibody detecting Paxillin in WB and IHC. Suitable for Human and Rat.

Quick Overview for Paxillin antibody (AA 29-33) (ABIN5689331)

Target: Paxillin (PXN)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Paxillin Antibody

Binding Specificity: AA 29-33

Specificity: This antibody detects endogenous level of total Paxillin protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Paxillin (Ab-31) antibody was raised against a peptide sequence around aa.29~33 (T-P-Y-S-Y) derived from Human Paxillin.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: Paxillin

Background: An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

Molecular Weight: 68 kDa

Gene ID: 5829

NCBI Accession: NP_001074324

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling