RING1 antibody (AA 35-39)

Catalog No. ABIN5689347

This Rabbit Polyclonal antibody specifically detects RING1 in WB. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for RING1 antibody (AA 35-39) (ABIN5689347)

Target: RING1 (Ring Finger Protein 1 (RING1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RING1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RING1 Antibody

Binding Specificity: AA 35-39

Specificity: This antibody detects endogenous level of total Ring1A protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Ring1A antibody was raised against a peptide sequence around aa.35~39 ( I-A-V-S-P) derived from Human Ring1A.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for RING1

Target: RING1 (Ring Finger Protein 1 (RING1))

Alternative Name: Ring1A

Background: Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

Molecular Weight: 58 kDa

Gene ID: 6015

NCBI Accession: NP_002922

UniProt: Q06587