PKM2 antibody (AA 405-409)

Catalog No. ABIN5689391

This anti-PKM2 antibody is a Rabbit Polyclonal antibody detecting PKM2 in WB. Suitable for Human.

Quick Overview for PKM2 antibody (AA 405-409) (ABIN5689391)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PKM2 Antibody

Binding Specificity: AA 405-409

Specificity: This antibody detects endogenous levels of total PKM2 protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: PKM2 antibody was raised against a peptide sequence around aa.405~409 (T-S-D-P-T) derived from Human PKM2.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2

Background: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Molecular Weight: 60 kDa

Gene ID: 5315

NCBI Accession: NP_872270

Pathways: Warburg Effect