14-3-3 zeta antibody (AA 656-60)

Catalog No. ABIN5689520

The Rabbit Polyclonal anti-14-3-3 zeta antibody (ABIN5689520) specifically detects 14-3-3 zeta in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for 14-3-3 zeta antibody (AA 656-60) (ABIN5689520)

Target: 14-3-3 zeta (YWHAZ)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 zeta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-14-3-3 zeta Antibody

Binding Specificity: AA 656-60

Specificity: This antibody detects endogenous level of total 14-3-3 zeta protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: 14-3-3 zeta (Ab-58) antibody was raised against a peptide sequence around aa.56~60 (R-S-S-W-R) derived from Human 14-3-3 zeta.

Application Details

Application Notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Alternative Name: 14 3 3 zeta

Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Molecular Weight: 28 kDa

Gene ID: 7534

NCBI Accession: NP_001129171

UniProt: P63104

Pathways: Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location