GFI1B antibody (AA 322-326)

Catalog No. ABIN5689567

The Rabbit Polyclonal anti-GFI1B antibody is suitable to detect GFI1B in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for GFI1B antibody (AA 322-326) (ABIN5689567)

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFI1B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GFI1B Antibody

Binding Specificity: AA 322-326

Specificity: This antibody detects endogenous level of total GFI1b protein.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: GFI1b antibody  was raised against a peptide sequence around aa.322~326 (H-R-E-S-Q) derived from Human GFI1b.

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details for GFI1B

Target: GFI1B (Growth Factor Independent 1B Transcription Repressor (GFI1B))

Alternative Name: GFI1b

Background: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Transcriptional repressor or activator depending on both promoter and cell type context, represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1, GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription, as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.

Molecular Weight: 38 kDa

Gene ID: 8328

NCBI Accession: NP_001128503

UniProt: Q5VTD9

Pathways: Cellular Response to Molecule of Bacterial Origin