Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Rubicon antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Rubicon antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
RUBICON antibody, 5330403K09 antibody, Kiaa0226 antibody, Rubicon antibody, mKIAA0226 antibody, RUN and cysteine rich domain containing beclin 1 interacting protein antibody, KIAA0226 antibody, RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein antibody, RUBCN antibody, KIAA0226 antibody, Rubcn antibody
Background
Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).