SMURF2 antibody (AA 140-190)

Catalog No. ABIN5690259

Product Details anti-SMURF2 Antibody

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Binding Specificity: AA 140-190

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMURF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.

Purification: SMURF2 antibody is affinity chromatography purified via peptide column.

Immunogen: SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2.

The immunogen is located within amino acids 140 - 190 of SMURF2.

Isotype: IgG

Application Details

Application Notes: SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 2 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SMURF2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: SMURF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for SMURF2

Target: SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

Alternative Name: SMURF2 (SMURF2 Products)

Synonyms: SMURF2 antibody, 2810411E22Rik antibody, AI558114 antibody, AI649275 antibody, RGD1310067 antibody, wu:fb82a11 antibody, wu:fc41c02 antibody, SMAD specific E3 ubiquitin protein ligase 2 antibody, SMAD specific E3 ubiquitin protein ligase 2 S homeolog antibody, SMURF2 antibody, smurf2 antibody, Smurf2 antibody, smurf2.S antibody

Background: SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3). Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3). SMURF2 associates constitutively with SMAD7 (4).

Molecular Weight: Predicted: 82 kDa

Observed: 85 kDa

Gene ID: 64750

NCBI Accession: NP_073576

UniProt: Q9HAU4