Vinculin antibody (AA 820-870)

Catalog No. ABIN5690318

The Rabbit Polyclonal anti-Vinculin antibody (ABIN5690318) specifically detects Vinculin in WB, ELISA, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Vinculin antibody (AA 820-870) (ABIN5690318)

Target: Vinculin (VCL)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Vinculin Antibody

Binding Specificity: AA 820-870

Specificity: Vinculin antibody is human, mouse and rat reactive. At least three isoforms of Vinculin are known to exist.

Purification: Vinculin antibody is affinity chromatography purified via peptide column.

Immunogen: Vinculin antibody was raised against a 23 amino acid peptide near the center of human Vinculin.

The immunogen is located within amino acids 820 - 870 of Vinculin.

Isotype: IgG

Application Details

Application Notes: Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Vinculin antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Vinculin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).

Molecular Weight: Predicted: 120 kDa

Observed: 117 kDa

Gene ID: 7414

NCBI Accession: NP_003364

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2