GM-CSF antibody (Biotin)

Catalog No. ABIN5690566

The Rabbit Polyclonal anti-GM-CSF antibody is suitable to detect GM-CSF in samples from Rat. It has been validated for WB and ELISA.

Quick Overview for GM-CSF antibody (Biotin) (ABIN5690566)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GM-CSF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-GM-CSF Antibody (Biotin)

Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Rat GM-CSF. Rat GM-CSF specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect Rat GM-CSF by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF.

Sandwich
To detect Rat GM-CSF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci's Polyclonal Anti-Rat GM-CSF (38-273) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GM-CSF.

Western Blot:

To detect Rat GM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Background: GM-CSF is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors.

Gene ID: 116630

UniProt: P48750

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin