APAF1 antibody (AA 1-464)

Catalog No. ABIN5690621

The Rat Monoclonal anti-APAF1 antibody (Clone 2E12) (ABIN5690621) specifically detects APAF1 in WB, IHC, ELISA, IP and ICC. The antibody is reactive with Human samples.

Quick Overview for APAF1 antibody (AA 1-464) (ABIN5690621)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 2E12

Product Details anti-APAF1 Antibody

Binding Specificity: AA 1-464

Specificity: Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.

Purity: > 95% (SDS-PAGE)

Immunogen: Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.

Isotype: IgG2a kappa

Application Details

Application Notes: Immunohistochemistry: (paraffin sections, 10 μg/mL) . Immunohistochemistry: (EM on frozen sections, see G. Hausmann, et al., (2000)) . Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for at least 1 year after receipt when stored at -20°C.

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: Apaf-1

Background: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Gene ID: 317

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity