Perforin 1 antibody (AA 98-534)

Catalog No. ABIN5690663

This anti-Perforin 1 antibody is a Rat Monoclonal antibody detecting Perforin 1 in WB, IHC, IP and ICC. Suitable for Mouse.

Quick Overview for Perforin 1 antibody (AA 98-534) (ABIN5690663)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: CB5-4

Product Details anti-Perforin 1 Antibody

Binding Specificity: AA 98-534

Purification: Perforin (monoclonal CB5.4) antibody was purified to ≥,95% in SDS-PAGE.

Immunogen: Perforin (monoclonal CB5.4) antibody was raised against a recombinant mouse perforin, amino acids 98-534.

Isotype: IgG2a

Application Details

Application Notes: Perforin antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL. This product can be used for detection of active human FasL by flow cytometry and western blot. Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1 % BSA, slides were incubated with 5 μ,g/mL of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC). Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 μ,g/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Perforin antibody can be stored at 4°C, stable for one year.

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin

Gene ID: 18646

UniProt: P10820

Pathways: Apoptosis, Caspase Cascade in Apoptosis