Tryptophan Hydroxylase 1 antibody

Catalog No. ABIN5690709

This Sheep Polyclonal antibody specifically detects Tryptophan Hydroxylase 1 in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for Tryptophan Hydroxylase 1 antibody (ABIN5690709)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This Tryptophan Hydroxylase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Tryptophan Hydroxylase 1 Antibody

Purification: Affinity Purified

Immunogen: TPH polyclonal antibody was raised against recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E.

Application Details

Application Notes: Tryptophan Hydroxylase antibody can be used for specific immunolabeling of the ~55k TPH protein in Western blots of human dorsal Raphe nucleus. Applications include Dot Blots (DB) Immunohistochemistry (IHC) and Western Blots (WB). The antibody has been used successfully with rat and human brain tissues in blot immunolabeling and immunohistochemical procedures. Based upon the relatively high degree of homology of tryptophan hydroxylase, the antibodies should cross-react with other mammalian species. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, DB and WB.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μLin 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per mL BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Target Details for Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Alternative Name: Tryptophan Hydroxylase

Background: Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis..

Molecular Weight: 55 kDa

Gene ID: 100009100

UniProt: P17290

Pathways: Feeding Behaviour