Cytokeratin 19 antibody (FITC)

Catalog No. ABIN5691090

This Mouse Monoclonal antibody specifically detects Cytokeratin 19 in FACS, ICC and IHC (fro). It exhibits reactivity toward Various Species.

Quick Overview for Cytokeratin 19 antibody (FITC) (ABIN5691090)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Various Species

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: RCK108

Product Details anti-Cytokeratin 19 Antibody (FITC)

Purpose: Mouse anti Cytokeratin 19/ Keratin 19, conjugated to FITC

Specificity: RCK108 reacts exclusively with cytokeratin 19 which is present in glandular-type epithelia and most carcinomas. Does not react with hepatocytes and hepatocellular carcinoma.

Characteristics: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Immunogen: RCK108 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal preparation of human bladder carcinoma cell line T24.

Isotype: IgG1

Application Details

Application Notes: "RCK108 is suitable for immunocytochemistry, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilutions for the different applications should be determined by titration. The recommended dilution is 1:10."

Restrictions: For Research Use only

Handling

Buffer: Each vial contains 1 mL FITC-conjugated anti cytokeratin 19 monoclonal antibody in PBS containing 0.1 % BSA, 0.09 % sodium azide. Approximately 100 tests.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: Cytokeratin 19/ Keratin 19

UniProt: P08727