Lamin A/C antibody (FITC)

Catalog No. ABIN5691105

This Mouse Monoclonal antibody specifically detects Lamin A/C in ICC, FACS and IHC (fro). It exhibits reactivity toward Various Species.

Quick Overview for Lamin A/C antibody (FITC) (ABIN5691105)

Target: Lamin A/C (LMNA)

Reactivity: Various Species

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin A/C antibody is conjugated to FITC

Application: Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 131C3

Product Details anti-Lamin A/C Antibody (FITC)

Purpose: Mouse anti Lamin A and C, conjugated to FITC

Specificity: 131C3 reacts with an epitope located between residues 319-566 in lamin A and C.

Characteristics: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.

Immunogen: 131C3 is a mouse monoclonal IgG1/k antibody derived by fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with purified rat liver lamins.

Isotype: IgG1

Application Details

Application Notes: 131C3 is suitable for immunocytochemistry, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilutions for the different applications should be determined by titration. The recommended dilution is 1:10.

Restrictions: For Research Use only

Handling

Buffer: Each vial contains 1 mL FITC-conjugated anti lamin A and C monoclonal antibody in PBS containing 0.1 % BSA, 0.09 % sodium azide. Approximately 100 tests.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Target Details for Lamin A/C

Target: Lamin A/C (LMNA)

Alternative Name: Lamin A and C

UniProt: P02545

Pathways: Apoptosis, Caspase Cascade in Apoptosis, ER-Nucleus Signaling, Protein targeting to Nucleus