POLL antibody (AA 307-407)

Catalog No. ABIN5692843

The Rabbit Polyclonal anti-POLL antibody has been validated for WB, ELISA and FACS. It is suitable to detect POLL in samples from Human, Rat and Mouse.

Quick Overview for POLL antibody (AA 307-407) (ABIN5692843)

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-POLL Antibody

Binding Specificity: AA 307-407

Purpose: Anti-DNA Polymerase lambda/POLL Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DNA Polymerase lambda/POLL Antibody Picoband® (ABIN5692843). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human DNA Polymerase lambda recombinant protein (Position: K307-N407).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: POLL polymerase (DNA ed), lambda". 2. García-Díaz M, Bebenek K, Kunkel TA, Blanco L (September 2001). "Identification of an intrinsic 5'-deoxyribose-5-phosphate lyase activity in human DNA polymerase lambda: a possible role in base excision repair". J. Biol. Chem. 276 (37): 34659-63.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for POLL

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Alternative Name: POLL

Background:

Synonyms: DNA polymerase lambda , Pol Lambda

Tissue Specificity: Expressed in a number of tissues. Abundant in testis.

Background: DNA polymerase lambda, also known as POLL, is a protein found in eukaryotes. In humans, it is encoded by the POLLA gene. POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, suggesting that POLL participates in short- and long-patch base excision repair. POLL is widely expressed, with abundant expression in pachytene spermatocytes of testis and in ovary.

Molecular Weight: 70 kDa

Gene ID: 27343

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response