POLL antibody (AA 420-500)

Catalog No. ABIN7214596

The Rabbit Polyclonal anti-POLL antibody is suitable to detect POLL in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for POLL antibody (AA 420-500) (ABIN7214596)

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-POLL Antibody

Binding Specificity: AA 420-500

Purpose: Rabbit Anti-DNA pol λ Polyclonal Antibody

Specificity: DNA pol λ Polyclonal Antibody detects endogenous levels of DNA pol λ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human DNA pol lambda at AA range: 420-500

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for POLL

Target: POLL (Polymerase (DNA Directed), lambda (POLL))

Alternative Name: DNA pol lambda

Background: POLL, DNA polymerase lambda, Pol Lambda, DNA polymerase beta-2, Pol beta2, DNA polymerase kappaPOLL encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described.

Molecular Weight: 63kD

Gene ID: 27343

UniProt: Q9UGP5

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response