NADPH Oxidase 4 antibody (C-Term)

Catalog No. ABIN5692899

This Rabbit Polyclonal antibody specifically detects NADPH Oxidase 4 in WB, IF and ICC. It exhibits reactivity toward Human and Monkey. It has been mentioned in 2+ publications

Quick Overview for NADPH Oxidase 4 antibody (C-Term) (ABIN5692899)

Target: NADPH Oxidase 4 (NOX4)

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NADPH Oxidase 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-NADPH Oxidase 4 Antibody

Binding Specificity: C-Term

Purpose: Anti-NADPH oxidase 4/NOX4 Antibody

Sequence: ILNTLLDDWK PYKLRRLYFI WVCRDIQSFR WFADLL

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NADPH oxidase 4/NOX4 Antibody. Tested in IF, ICC, WB applications. This antibody reacts with Human, Monkey.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NADPH oxidase 4, which shares 97.2% amino acid (aa) sequence identity with both mouse and rat NADPH oxidase 4.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunocytochemistry/Immunofluorescence, 5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-NADPH Oxidase 4 antibody (ABIN5692899)

Liu, Hong, Li, Ren, Wang, Xu, Shi, Xu: "A Cross Talk Between BRG1 and Males Absent on the First Contributes to Reactive Oxygen Species Production in a Mouse Model of Nonalcoholic Steatohepatitis." in: Antioxidants & redox signaling, (2018) (PubMed).

Meyer, Fredette, Daniel, Sharma, Amann, Arterburn, Barton, Prossnitz: "Obligatory role for GPER in cardiovascular aging and disease." in: Science signaling, Vol. 9, Issue 452, pp. ra105, (2017) (PubMed).

Target Details for NADPH Oxidase 4

Target: NADPH Oxidase 4 (NOX4)

Alternative Name: NOX4

Background:

Background: NADPH oxidase 4 is an enzyme that in humans is encoded by the NOX4 gene, and is a member of the NOX family of NADPH oxidases. This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.

Gene Full Name: NADPH oxidase 4

Molecular Weight: 65 kDa

Gene ID: 50507

UniProt: Q9NPH5

Pathways: Carbohydrate Homeostasis, Smooth Muscle Cell Migration