14-3-3 sigma/SFN antibody (AA 1-248)

Catalog No. ABIN5693156

This anti-14-3-3 sigma/SFN antibody is a Rabbit Polyclonal antibody detecting 14-3-3 sigma/SFN in WB, IHC, ELISA, IF, ICC and FACS. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for 14-3-3 sigma/SFN antibody (AA 1-248) (ABIN5693156)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 sigma/SFN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-14-3-3 sigma/SFN Antibody

Binding Specificity: AA 1-248

Purpose: Anti-14-3-3 sigma/SFN Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-14-3-3 sigma/SFN Antibody Picoband® (ABIN5693156). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
Immunofluorescence, 2 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Leffers, H., Madsen, P., Rasmussen, H. H., Honore, B., Andersen, A. H., Walbum, E., Vandekerckhove, J., Celis, J. E.Molecular cloning and expression of the transformation sensitive epithelial marker stratifin: a member of a protein family that has been involved in the protein kinase C signalling pathway.J. Molec. Biol. 231: 982-998, 1993. 2. Urano, T., Saito, T., Tsukui, T., Fujita, M., Hosoi, T., Muramatsu, M., Ouchi, Y., Inoue, S. Efp targets 14-3-3-sigma for proteolysis and promotes breast tumour growth.Nature 417: 871-875, 2002. 3. Wilker, E. W., van Vugt, M. A. T. M., Artim, S. A., Huang, P. H., Petersen, C. P., Reinhardt, H. C., Feng, Y., Sharp, P. A., Sonenberg, N., White, F. M., Yaffe, M. B.14-3-3-sigma controls mitotic translation to facilitate cytokinesis.Nature 446: 329-332, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-14-3-3 sigma/SFN antibody (ABIN5693156)

Yang, Cheung, Peng, He, Wu, Xie, Jiang, Huang, Chen, Lin, Kung: "Makorin-2 is a neurogenesis inhibitor downstream of phosphatidylinositol 3-kinase/Akt (PI3K/Akt) signal." in: The Journal of biological chemistry, Vol. 283, Issue 13, pp. 8486-95, (2008) (PubMed).

Target Details for 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Alternative Name: SFN

Background:

Synonyms: 14-3-3 protein sigma, Epithelial cell marker protein 1, Stratifin, SFN, HME1

Tissue Specificity: Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

Background: Stratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

Molecular Weight: 28 kDa

Gene ID: 2810

UniProt: P31947

Pathways: p53 Signaling, Myometrial Relaxation and Contraction