ADHFE1 antibody

Catalog No. ABIN5698812

The Rabbit Polyclonal anti-ADHFE1 antibody (ABIN5698812) specifically detects ADHFE1 in ELISA, IHC and WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ADHFE1 antibody (ABIN5698812)

Target: ADHFE1 (Alcohol Dehydrogenase, Iron Containing, 1 (ADHFE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADHFE1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-ADHFE1 Antibody

Purpose: ADHFE1 antibody

Immunogen: alcohol dehydrogenase, iron containing, 1

Isotype: IgG

Application Details

Application Notes: WB :1:500-1:5000,IHC:1:20-1:200

Comment:

rat kidney tissue were subjected to SDS PAGE followed by western blot with FNab00167(ADHFE1 antibody) at dilution of 1:1000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ADHFE1

Target: ADHFE1 (Alcohol Dehydrogenase, Iron Containing, 1 (ADHFE1))

Alternative Name: ADHFE1

Background:

Synonyms: Hydroxyacid-oxoacid transhydrogenase, mitochondrial (HOT)|Alcohol dehydrogenase iron-containing protein 1 (ADHFe1)|Fe-containing alcohol dehydrogenase|ADHFE1

Background: Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate(GHB) to succinic semialdehyde(SSA) coupled to reduction of 2-ketoglutarate(2-KG) to D-2-hydroxyglutarate(D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate(L-3-OHB) are also substrates for HOT with 10-fold lower activities.

Molecular Weight: 50kDa

Gene ID: 137872

UniProt: Q8IWW8