ADHFE1 antibody

Catalog No. ABIN5698813

This Rabbit Polyclonal antibody specifically detects ADHFE1 in ELISA, IHC, WB and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ADHFE1 antibody (ABIN5698813)

Target: ADHFE1 (Alcohol Dehydrogenase, Iron Containing, 1 (ADHFE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADHFE1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-ADHFE1 Antibody

Purpose: ADHFE1 antibody

Immunogen: alcohol dehydrogenase, iron containing, 1

Isotype: IgG

Application Details

Application Notes: WB :1:1000-1:10000,IHC:1:20-1:200,IP:1:200-1:1000

Comment:

mouse heart tissue were subjected to SDS PAGE followed by western blot with FNab00168(ADHFE1 antibody) at dilution of 1:1000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ADHFE1

Target: ADHFE1 (Alcohol Dehydrogenase, Iron Containing, 1 (ADHFE1))

Alternative Name: ADHFE1

Background:

Synonyms: Hydroxyacid-oxoacid transhydrogenase, mitochondrial (HOT)|Alcohol dehydrogenase iron-containing protein 1 (ADHFe1)|Fe-containing alcohol dehydrogenase|ADHFE1

Background: Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate(GHB) to succinic semialdehyde(SSA) coupled to reduction of 2-ketoglutarate(2-KG) to D-2-hydroxyglutarate(D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate(L-3-OHB) are also substrates for HOT with 10-fold lower activities.

Molecular Weight: 50kDa

Gene ID: 137872

UniProt: Q8IWW8